Composite
Part:BBa_K1339004
Designed by: Madoka NAGATA Group: iGEM14_Tokyo-NoKoGen (2014-10-02)
RBS-otsB-RBS-otsA-dT
RBS-otsB-RBS-otsA-dT
This part contains the ORFs of the otsB and otsA genes from Escherichia coli K-12, each following the RBS. A double terminator is located downstream of otsA. otsB encodes trehalose-6-phosphate phosphatase and otsA encodes trehalose-6-phosphate synthase, which together produce trehalose from UDP-glucose and D-glucose-6-phsphate.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 473
Illegal BamHI site found at 1228 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
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