Composite

Part:BBa_K1339004

Designed by: Madoka NAGATA   Group: iGEM14_Tokyo-NoKoGen   (2014-10-02)

RBS-otsB-RBS-otsA-dT

RBS-otsB-RBS-otsA-dT

This part contains the ORFs of the otsB and otsA genes from Escherichia coli K-12, each following the RBS. A double terminator is located downstream of otsA. otsB encodes trehalose-6-phosphate phosphatase and otsA encodes trehalose-6-phosphate synthase, which together produce trehalose from UDP-glucose and D-glucose-6-phsphate.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 473
    Illegal BamHI site found at 1228
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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