Composite

Part:BBa_K1336007

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-10)

LacI Expression Cassette with BsDyP gene

BsDyP (BSU38260; accession number, NC_000964.3) gene (1251bp) from Bacillus subtilis, found to have been effective in degrading lignin and azo dyes, in an oxidative process. The range of substrates for this enzyme seems to be rather wide, including azo-dyes, phenolic compounds and other aromatics, as well as manganese and ferrous ions. The range of substrates and the mode of action of this enzyme suggests that it could be classified as a bacterial lignin peroxidase. This gene is ligated to a LacI expression cassette, IPTG inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1741
    Illegal BsaI site found at 1750
    Illegal BsaI site found at 2716
    Illegal SapI site found at 2146
    Illegal SapI site found at 2504
    Illegal SapI.rc site found at 2660


[edit]
Categories
Parameters
None