Composite

Part:BBa_K1336006

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)

LacI Expression Cassette with ispB RC gene

An antisense fragment designed using the sense/coding strand of the ispB [1] gene as a method for gene knockdown (i.e. as RNAi) of a crucial stage in the production of the side-chains of isoprene quinones within E. coli metabolic processes. At the 5' end, there is also a LacI expression cassette [2] IPTG-inducible promoter to drive ispB expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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