Coding

Part:BBa_K1336003

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)

Dye-decolourising peroxidase from Bacillus subtilis

BsDyP (BSU38260; accession number, NC_000964.3) gene (1251bp) from Bacillus subtilis, found to have been effective in degrading lignin and azo dyes, in an oxidative process. The range of substrates for this enzyme seems to be rather wide, including azo-dyes, phenolic compounds and other aromatics, as well as manganese and ferrous ions. The range of substrates and the mode of action of this enzyme suggests that it could be classified as a bacterial lignin peroxidase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 97
    Illegal BsaI site found at 106
    Illegal BsaI site found at 1072
    Illegal SapI site found at 502
    Illegal SapI site found at 860
    Illegal SapI.rc site found at 1016


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Categories
Parameters
None