Coding

Part:BBa_K1336002

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)


Spore coat protein, laccase, from Bacillus subtilis

CotA gene (1542bp) coding for an enzyme from Bacillus subtilis that is used to degrade and decolourise azo dyes, through an oxidative process. This part was improved by <a href="https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2018&group=SHSBNU_China">Team SHSBNU_China</a> as <a href="https://parts.igem.org/Part:BBa_K2684000">BBa_K2684000</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 867
    Illegal XbaI site found at 1482
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 867
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 867
    Illegal XbaI site found at 1482
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 867
    Illegal XbaI site found at 1482
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1509
    Illegal SapI.rc site found at 286

improvements

this part was improved as BBa_K2684000 by Team SHSBNU_China

Functional Parameters

[edit]
Categories
Parameters
None