Coding

Part:BBa_K1336001

Designed by: Yan-Kay Ho   Group: iGEM14_UCL   (2014-10-06)

Azoreductase 1B6 (Heat-stable Azoreductase mutant)

A gene (612bp) coding for a non-specific enzyme from Pseudomonas putida that is used to degrade a double nitrogen (N=N) azo bond, as found in azo dyes. It is heat-stable AzoR [1] mutant .

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 89
    Illegal PstI site found at 170
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None