Composite

Part:BBa_K1334026

Designed by: Haoyuan Sun   Group: iGEM14_WHU-China   (2014-09-29)


IPTG inducible E7 protein and GFP generator

K1334026 is composed of four main parts. A constitutive lacI repressor generator(J23106,P0412), an inducable promoter which can be activated by adding IPTG(R0011), an E7 lysis protein which can result in the lysis of E.coli if being expressed, and a GFP reporter cotranscribed with E7. This reconstructed biobrick can be used to prove the function of E7. When IPTG is added to the mixture, the growth rate of E.coli will drop, and a large amount of GFP can be detected in the supernatant of the bacterium solution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2085


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