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Signalling

Part:BBa_K1334020

Designed by: Qiushuang Wu   Group: iGEM14_WHU-China   (2014-09-27)

a signal complex to test whether the lux system works

When luxR is activated,the promoter plux will be activated,the expression of RFP is up_regulated.this device is used to test the function and sensitivity of the lux system


Usage and Biology

We transform this part which is constructed in the pSB1C3 backbone into the E.coli strain DH5a. After exogenous AHL (3OC6HSL ,final concentration:100nM)stimulation, we detect the relative fluorescence intensity of RFP. The following figures shows that the lux system works well.

Figure The function test of our part BBa K1334020 in which the relative fluorescence reflect that the device works.png
           Figure 1 The function test of our part BBa_K1334020 in which the relative fluorescence reflect that the device works well.


Figure 12 A picture which shows the color change of the bacteria after AHL (3OC6HSL) stimulation2.png
                       Figure 2 A picture which shows the color change after AHL (3OC6HSL) stimulation.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1686
    Illegal AgeI site found at 1798
  • 1000
    COMPATIBLE WITH RFC[1000]


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