Translational_Unit

Part:BBa_K1334019

Designed by: Qiushuang Wu   Group: iGEM14_WHU-China   (2014-09-27)

promoter+RBS+FDH+terminator

we assembly the promoter J23102,RBS J61100,FDH with a flag tag and a terminator B0015 together.Then,with the antibody of flag tag,we are able to detect the expression of FDH easily

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1321
    Illegal BglII site found at 1560
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1016
    Illegal AgeI site found at 1835
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None