Coding

Part:BBa_K1334014

Designed by: Haoyuan Sun   Group: iGEM14_WHU-China   (2014-09-27)

formate dehydrogenase(FDH) from Escherichia.coli DH5a with a Flag-tag

FDH from E.coli is formate dehydrogenase,which can oxidize formate to carbon dioxide in the presence of NAD+ Sequence and Features.We add a Flag-tag so the expression can be easily detected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1260
    Illegal BglII site found at 1499
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1774
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None