Regulatory

Part:BBa_K1334002

Designed by: Haoyuan Sun   Group: iGEM14_WHU-China   (2014-09-27)

HxlR+P-formaldehyde

A right promoter which can sense formaldehyde.There is a left promoter which can erpress the HxlR protein.The HxlR protein can enhance the function of the P-formaldehyde.


Usage and Biology

A gfp reporter gene was added downstream so that we can know whether the P-formaldehyde works or not in the presence of formaldehyde by detecting the relative fluorescence intensity.

Figure3 A GFP is added downstream of the formaldehyde promoter.jpg

Figure 1 A GFP reporter is added downstream of the formaldehyde promoter.

We incubated E.coli strain DH5a with the above plasmid (P-formaldehyde plus GFP)and stimulated the experimental group with 1mM formaldehyde when the OD600 value reached approximately one, which means E.coli comes into mid-log phase. And then we collected bacteria at certain time to measure the fluorescence and the absorbance.

From the Figure 2, we can see significant increase in relative fluorescence intensity after formaldehyde stimulation, which shows that our coloration system works well.

The Growth Condition curve in Figure 3 shows that the difference of the fluorescence/absorbance is not generated by the absorbance difference.

Figure 4 The function test of the formaldehyde promoter which shows our part BBa K1334002 works well 2.png

Figure 2 The function test of the formaldehyde promoter which shows our part BBa_K1334002 works well。

Figure 5 The growth condition of E.coli strain DH5a after formaldehyde stimulation.png

Figure 3 The growth condition of E.coli strain DH5a after formaldehyde stimulation.


Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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