Part:BBa_K1333107

umuc

The UmuC is involved in the SOS DNA repair process.

Usage and Biology

When DNA encounters severe damage which may leads to critical problem of the cell, the most prior task is to stabilize the DNA as soon as possible, even at the cost of generate mutation. SOS bypass is an emergency DNA repair method of cells. The functional enzyme of SOS is polymerase V. When the external signal ,like UV, activate the RecA coprotease activity, it can help the lexA gene to cleave itself, which will result in several genes to be induced. UmuD and UmuC are two of the induced genes. The product of UmuD gene will be cleaved by a proteinase and turn into UmuD’. The complex of UmuD’ and UmuC has DNA polymerase activity, which is referred as polymerase V.

SOS error prone bypass

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 177
  Illegal BamHI site found at 1184
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 75
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 265