Coding

Part:BBa_K1333101

Designed by:   Group: iGEM14_SYSU-China   (2014-09-24)

RBS DNAQ

DNAQ is the proofreading subunit of E.coli DNA polymerase III.


Usage and Biology

DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication.

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutation

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 64
    Illegal AgeI site found at 553
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None