Part:BBa_K1332013

SmtA semi-permanent generator

This generator is capable of synthesizing SmtA (BBa_K519010). This generator consists of a mRNA circularization device (5’ side)(BBa_K1332008), SmtA (BBa_K519010) and a mRNA circularization device (3’ side)(endless translation))(BBa_K1332009). A mRNA is circular, so translation continues semi-permanently. SmtA is a metallothionein that can catch heavy metal ions such as Zn²⁺.

The efficacy of a long-chain SmtA

Figure1. SDS-PAGE of the long chain protein of SmtA

I was able to confirm more than 250 kDa of SmtA. Because the metallothionine is the simple structure protein which only adsorbs metal such as Zn, the Cd, even if it becomes a long chain, it's not lose activity. We measured the activity but didn't get a reliable data.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 545
  
• 1000
  COMPATIBLE WITH RFC[1000]