Part:BBa_K1332012

A TTHA0715 with sequence cut by thrombin semi-permanent generator

This generator is capable of synthesizing TTHA0715 (with amino acid sequence cut by thrombin). This generator consists of a mRNA circularization device (5´ side), TTHA0715 (with amino acid sequence cut by thrombin) and a circularization device (3’ side). A mRNA is circular, so translation continues semi-permanently. TTHA0715 is a cold shock protein and ABC transporter ATP-binding protein. The use of this device is to confirm the existence of the long-chain protein that is made by a circular mRNA. But this part didn’t work.

Existence proof of a long-chain protein

This part didn’t work. We think that the reason for the part’s dysfunction is the GC content of the sequence of TTHA0715. We think that the GC content is too high, so it affects adversely a circularization of mRNA or the translation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]