Coding

Part:BBa_K1332002

Designed by: Kenta Nomura   Group: iGEM14_Gifu   (2014-09-26)

Histidine tag (8 AA) and RFP (without stop codon)

A Histidine tag is attached to a RFP that is coded by this part. So the RFP can be purified by immobilized metal ions affinity chromatography.

!!Caution!!

A stop codon in this part is removed. Therefore, this part doesn’t have an ability of an usual protein coding part. The combination of this part and the mRNA circularization device is capable of generating the RFP (+histidine tag) polymer. The part to express it is registered (BBa_K1332011).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 584
    Illegal AgeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None