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Coding

Part:BBa_K1332001

Designed by: Kenta Nomura   Group: iGEM14_Gifu   (2014-09-25)

Histidine tag (8 AA) and RFP

This part codes for a RFP(BBa_E1010). A histidine tag is attached to a RFP that is coded by this part. So the RFP can be purified by immobilized metal ions affinity chromatography.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 584
    Illegal AgeI site found at 696
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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