Generator

Part:BBa_K133124

Designed by: Jan Lonzaric   Group: iGEM08_Slovenia   (2008-10-25)

CMV-SS-HA-UreB-Histop

Construct coding for Urease B with Ha-tag and His-tag, secreted from the cells because of the signaling sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1012
    Illegal AgeI site found at 2190
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 898


[edit]
Categories
//chassis/eukaryote
Parameters
None