Intermediate

Part:BBa_K133118

Designed by: Katja Kolar   Group: iGEM08_Slovenia   (2008-10-29)

CD4sh-(TIR)TLR3-Histop

An improvement of the bibrick BBa_I712084 with the addition of His-tag. Can be used for C-terminal fusions, because it contains a stop codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1141
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 975
    Illegal SapI.rc site found at 63


[edit]
Categories
Parameters
None