Intermediate

Part:BBa_K133116

Designed by: Katja Kolar   Group: iGEM08_Slovenia   (2008-10-23)

UreB-Histop

Urease B (UreB) is one of the main H. pylori antigens. It is a subunit of enzyme urease, composed of A and B subunit that neutralizes the pH around the microorganism by decomposing urea into ammonia and carbon dioxide, thus enabling H. pylori to survive in the acidic gastric environment. Application of this antigen should elicit production of neutralizing antibodies thus inhibiting enzymatic activity of urease, which could lead to the eventual eradication of bacteria.

In this construct UreB is fused with His-tag and a stop codon for C-terminal fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1013
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 235
    Illegal AgeI site found at 1413
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 121


[edit]
Categories
Parameters
None