Intermediate

Part:BBa_K133114

Designed by: Jan Lonzaric   Group: iGEM08_Slovenia   (2008-10-25)

multiHP-Histop

Synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. UreB epitope comprises AA 314-339 of Helicobacter pylori urease B, VacA AA 368-487 of vacuolating cytotoxin A of Helicobacter pylori and HpaA AA 134-146 of Helicobacter pylori. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction. Some of them were already shown to elicit antibody production, thus our combination broadens a repertoire of activated B-cells.

In this part, the multiepitope is fused with His-tag and contains a stop codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None