Intermediate

Part:BBa_K133106

Designed by: Jan Lonzaric   Group: iGEM08_Slovenia   (2008-10-25)

(TMTIR)TLR9-GFP-Histop

Transmembrane and cytosolic (TIR) domain of human Toll-like receptor 9 fused with GFP reporter gene and His-tag. Can only be used as C-terminal part of a fusion, because it contains a stop codon.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 382


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Categories
Parameters
None