Intermediate

Part:BBa_K133103

Designed by: Katja Kolar   Group: iGEM08_Slovenia   (2008-10-25)

CMV-SS-HA-(TMTIR)TLR4-Histop

Transmembrane (TM) and cytosolic (TIR) domains of human TLR4 under control of constitutive promotor CMV and equiped with HA-tag on N-terminus and His-tag on C-terminus.

Construct codes for a transmembrane protein expressed on the cell surface. It is possible to lable the protein with fluroscently labeled antibodies against the HA tag on the outside of the cell or against the His-tag in the cytosol.

This part was designed to compare its activity with the activity of part BBa_K113112 that contains a multiepitope antigen in addition to the TLR4 TM and TIR domains.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None