Coding

Part:BBa_K1331007

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-26)

Modified rhlRI coding sequence from Pseudomonas aeruginosa SQ6

This part encodes both rhlR and rhlI. It is composited with BBa_K1331002 and BBa_K1331003.

Usage and Biology

The sequence we provide here includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.

The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 863
    Illegal BamHI site found at 344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1126


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Categories
Parameters
None