Coding
Part:BBa_K1331006
Designed by: Tianyi Huang Group: iGEM14_Nankai (2014-09-26)
Modified rhlAB coding sequence from Pseudomonas aeruginosa SQ6
This is a composite part made of BBa_K1331001 and BBa_K1331004.
Usage and Biology
This part encodes both the subunit A and B, and together make the functional rhamnosyltransferase I, the essential enzyme for rhamnolipid synthesis. This sequence includes both the coding sequence of rhlA, rhlB and the whole noncoding sequence between rhlA and rhlB. Preserving this noncoding sequence here is to ensure the accurate expression of rhamnosyltransferase I. Besides, a part of downstream noncoding sequence of rhlB is also included for composition with rhlRI, see our part BBa_K1331005.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
Illegal XhoI site found at 2099 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 992
Illegal NgoMIV site found at 1713
Illegal NgoMIV site found at 1826 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 294
Illegal BsaI site found at 1342
Illegal BsaI.rc site found at 478
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Categories
Parameters
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
//cds/enzyme
//chassis/prokaryote/ecoli
None |