Coding

Part:BBa_K1331004

Designed by: Tianyi Huang   Group: iGEM14_Nankai   (2014-09-25)

Modified rhlB coding sequence from Pseudomonas aeruginosa SQ6


This is an improvement of BBa_K317999 designed by iGEM10_Tokyo-NoKoGen. Two mutations have been done to remove the PstI restriction sites (at 790 and 904) to meet BioBrick RFC[10] requirement without changing the amino acid sequence.

Usage and Biology

The sequence we provide here includes the whole coding sequence as well as a part of upstream noncoding sequence and a part of downstream noncoding sequence of rhlB.

RhlB together with rhlA encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa. This part encodes rhamnosyltransferase I subunit B, and our part BBa_K1331001 encodes subunit A.

The noncoding sequence included in this part is for composition with rhlA and with rhlR. See our composite parts rhlAB (BBa_K1331006) and rhlABRI (BBa_K1331005).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 37
    Illegal NgoMIV site found at 758
    Illegal NgoMIV site found at 871
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387


[edit]
Categories
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
Parameters
None