Part:BBa_K133094

CMV-SS-HA-UreB-CD4ecto-(TMTIR)TLR9-Histop

The most well known Helicobacter pylori antigen, urease B, fused with the extracellular domain of human CD4 and the transmembrane (TM) and cytosolic (TIR) domain of human Toll-like receptor 9. Construct is under control of constitutive promotor CMV and contains His-tag on C-terminus and HA-tag on N-terminus (after the CD4 signaling sequence).

Construct codes for a transmembrane protein expressed on the endosomal membrane. The protein can be visualized by staining with fluroscently labeled antibodies against the HA-tag inside the endosomes or against the His-tag in the cytosol.

The CD4 ecto domain enables constitutive homodimerization of the protein and thus activation of the TIR domain. This activation triggers transcription of genes under the NFkB promoter and stimulates the innate immune response. After the activation the antigen is processed in the endosome and short peptides are presented on MHC II molecules.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 3020
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1790
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1012
  Illegal AgeI site found at 2190
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3467
  Illegal BsaI site found at 3995
  Illegal SapI.rc site found at 898
  Illegal SapI.rc site found at 2555