Composite

Part:BBa_K133092

Designed by: Katja Kolar   Group: iGEM08_Slovenia   (2008-10-29)

CMV-SS-HA-CD4ecto-(TMTIR)TLR3stop

Extracellular domain of human CD4 fused with the transmembrane (TM) and cytosolic (TIR) domain of human Toll-like receptor 3. Construct is under control of constitutive promotor CMV and HA-tag on N-termuninus (after the CD4 signaling sequence).

Construct codes for a transmembrane protein expressed on the endosomal membrane. It is possible to lable the protein with fluroscently labeled antibodies against the HA tag in the endosomes.

The CD4 ecto domain enables constitutive homodimerization of the protein and thus activation of the TIR domain. This activation triggers transcription of genes under the IFNb promoter and stimulates the innate immune response.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1754
    Illegal SapI.rc site found at 842


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