Composite

Part:BBa_K133091

Designed by: Katja Kolar   Group: iGEM08_Slovenia   (2008-10-29)

CMV-SS-HA-CD4ecto-(TMTIR)TLR9-Histop

Extracellular domain of human CD4 fused with the transmembrane (TM) and cytosolic (TIR) domain of human Toll-like receptor 9. Construct is under control of constitutive promoter CMV and contains His-tag on C terminus and HA-tag on N termuninus (after the CD4 signaling sequence).

Construct codes for a transmembrane protein expressed on the endosomal membrane. It is possible to lable the protein with fluroscently labeled antibodies against the HA tag in the endosomes or against the His-tag in the cytosol.

The CD4 ecto domain enables constitutive homodimerization of the protein and thus activation of the TIR domain. This activation triggers transcription of genes under the NFkB promoter and stimulates the innate immune response.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1307
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1754
    Illegal BsaI site found at 2282
    Illegal SapI.rc site found at 842


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