Part:BBa_K133043
tetR-RBS-CF-RGD-Histop
TetR repressible promoter with ribosomal binding site.
CF – Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.
Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1248
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 805
Illegal BamHI site found at 1316 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 342
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1599
None |