Part:BBa_K133039

T7-CF-UREB-RGD-Histop

This part is ligated into a plasmid with T7 promotor. The plasmid was then transformed into cells with T7 Phage. This enabled the expression of the protein.

The part consists of:

1. Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production.

2. UreB – beta subunit of H. pylori urease that we used as an antigen

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1207
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 764
  Illegal BamHI site found at 1275
  Illegal BamHI site found at 2564
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 301
  Illegal AgeI site found at 1786
  Illegal AgeI site found at 2964
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3271
  Illegal SapI.rc site found at 1672