Part:BBa_K133031

HpaA-CF-RGD-Histop

This part is composed of

1. HpaA, which is coding H.pylori antigen, putative neuraminyllactose-binding hemagglutinin homolog

2. Chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production..

3.Because the protein was designed to be produced, we additionally added RGD-Hisstop tag.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 139
  Illegal NheI site found at 1993
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 170
  Illegal BglII site found at 266
  Illegal BamHI site found at 1550
  Illegal BamHI site found at 2061
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1087
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2344