Intermediate

Part:BBa_K133029

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

HpaA-multiHP-RGD-Histop

This part is composed of

1. HpaA, which is coding H.pylori antigen, putative neuraminyllactose-binding hemagglutinin homolog

2. synthetic multiepitope, comprising the H. pylori urease B epitope, a vacuolating cytotoxin A (Vac) epitope and an adhesin A (HpaA) epitope. B-cell epitopes were designed using available 3D models or structures, which enabled more accurate prediction.

3.Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 139
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 170
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1326


[edit]
Categories
Parameters
None