Part:BBa_K133024

CMV-ss-CF-UreB-RGD-Histop

We have used CMV promotor, because it is promotor for constitutive expression in mammalian cells.

SS is a CD4 signal peptide for trafficking to the cellular membrane.

The rest of the part consists of:

1. CF - chimeric fusion protein, which retains the central, most antigenic segment of H. pylori (hypervariable region) flagellin FlaA, while replacing the N- and C-terminal segment by the FliC flagellin from E. coli, which is responsible for the activation of TLR5 (176 AA from the N-terminal and 99 AA from the C-terminal). Thus, combining the right parts of flagellin of E. coli with that of H. pylori enables activation of innate immunity - a characteristic that flagellin of H. pylori alone does not posess - while presenting the antigen of H. pylori for antibody production..

2. UreB, which is beta subunit of H. pylori urease that we used as an antigen

3. Since the protein was designed to be produced, we additionally added RGD-Hisstop tag.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1948
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1505
  Illegal BamHI site found at 2016
  Illegal BamHI site found at 3305
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1042
  Illegal AgeI site found at 2527
  Illegal AgeI site found at 3705
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 4012
  Illegal SapI.rc site found at 2413