Coding

Part:BBa_K1328001

Designed by: Tianyi Li   Group: iGEM14_Tsinghua   (2014-10-09)


HXT1-CMVmini-EGFP-tADH1

This is a composite part containing a glucose-sensitive promoter (BBa_K1328000) and a EGFP reporter with a tADH1 transcription terminator.

S. cerevisiae cells transformed with this part was cultured in glucose-free medium with glycerol as carbon-source for 8 hrs. They were then cultured for an additional hour with varying glucose levels (0, 5, 15, 25 mmol/L). A increasing level of GFP expression was observed with increasing levels of glucose concentration, in both microscopy and flow cytometry observations.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 516
    Illegal PstI site found at 213
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 516
    Illegal PstI site found at 213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 516
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 516
    Illegal PstI site found at 213
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 516
    Illegal PstI site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None