Part:BBa_K1326003

Plac + POR + DVR1 + ChlP + ChlG

Composite part entailing genes at the end of the chlorophyll biosynthetic pathway, responsible for the construction of the chlorophyll molecule. Light-dependent protochlorophyllidereductase (POR), a chloroplast precursor, uses NADPH and light to convert protochlorophyllide to chlorophyllide. DVR1 is a chloroplast transit peptide. Plant geranylgeranylhydrogenase (ChlP) reduces free geranylgeranyldiphosphate to phytildiphosphate; this leads to the construction of the chlorophyll side-chain Chlorophyllsynthetase (ChlG) is a catalyst for the esterification of chlorophyllide with phytyl-pyrophosphate to make the chlorophyll molecule.


Figure 1: Operon 3 in the chlorophyll biosynthesis pathway.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 808
  Illegal BglII site found at 4090
  Illegal BglII site found at 4579
  Illegal BamHI site found at 1571
  Illegal BamHI site found at 3859
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 347
  Illegal AgeI site found at 398
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2461
  

Functional Parameters

Figure 1: Single and double restriction digests of Operon 3. Total plasmid length is 7.10 kB, as can be seen by the dark band in the single digest lane. Double digest lane shows the operon fragment at 4.90 kB, and the BioBrick plasmid backbone at 2.20.