Part:BBa_K1326002

Plac + CTH1 + YCF54 + Plasto + ChlM

Composite part incorporating the genes comprising the middle third of the chlorophyll biosynthetic pathway. CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule. Plastocyanin is a copper-containing chloroplast precursor protein. Copper is involved in electron transfer on plastocyanin's functional site between cytochrome f (from cyctochrome b6f complex, from photosystem II) and P700+ (from photosystem I). ChlM is shorthand for Magnesium-protoporphyrin O-methyltransferase, which catalyses the methylation of magnesium protoporphyrin IX., frming magnesium protoporphyrin IX monomethylester.

Figure 1: Operon 2 involved in chlorophyll biosynthesis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 513
  Illegal BglII site found at 621
  Illegal BglII site found at 2409
  Illegal BamHI site found at 1476
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2966
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1638
  Illegal BsaI.rc site found at 311
  Illegal BsaI.rc site found at 467
  Illegal BsaI.rc site found at 1088
  Illegal BsaI.rc site found at 1947
  Illegal BsaI.rc site found at 2070
  

Functional Parameters

Figure 1: Single and double restriction digests of Operon 2. Single digest shows entire plasmid length at 5.48 kB. Double digest shows bands at 3.28 and 2.20 kB, for the operon 2 fragment length and BioBrick plasmid backbone respectively.