Plac + CTH1 + YCF54 + Plasto + ChlM
Composite part incorporating the genes comprising the middle third of the chlorophyll biosynthetic pathway.
CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule.
Plastocyanin is a copper-containing chloroplast precursor protein. Copper is involved in electron transfer on plastocyanin's functional site between cytochrome f (from cyctochrome b6f complex, from photosystem II) and P700+ (from photosystem I).
ChlM is shorthand for Magnesium-protoporphyrin O-methyltransferase, which catalyses the methylation of magnesium protoporphyrin IX., frming magnesium protoporphyrin IX monomethylester.
Figure 1: Operon 2 involved in chlorophyll biosynthesis.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 513
Illegal BglII site found at 621
Illegal BglII site found at 2409
Illegal BamHI site found at 1476
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 2966
- 1000Illegal BsaI site found at 1638
Illegal BsaI.rc site found at 311
Illegal BsaI.rc site found at 467
Illegal BsaI.rc site found at 1088
Illegal BsaI.rc site found at 1947
Illegal BsaI.rc site found at 2070
Figure 1: Single and double restriction digests of Operon 2. Single digest shows entire plasmid length at 5.48 kB. Double digest shows bands at 3.28 and 2.20 kB, for the operon 2 fragment length and BioBrick plasmid backbone respectively.