Device

Part:BBa_K1325016

Designed by: Zongzhi Lv   Group: iGEM14_BIT-China   (2014-10-02)

small RNAs regulatory system In this device, we can construct artificial sRNAs that can regulate desired genes in E.coli , screen sRNAs and obtain the results of initiation efficiency of sRNAs. Small RNAs regulatory system (BBa_K1325016) is composed of box1 and box2. The model figure is below.

BIT-China-123456.png

Box1 consists of promoter, anti-target sequence, MicC gene and terminators.Box2 consists of promoter, RBS, target sequence, lacZ gene. We are add target-gene and anti-target-gene sequence to small RNAs regulatory system by One-step Site-directed Mutagenesis. Basic on this device, we can design many kinds of sRNAs that we need. Here we design two kinds of sRNAs, including sRNA-sicA (BBa_K1325012) and sRNA-ipgC (BBa_K1325013). There are results (Fig.1)

BIT-China-1234567.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 129
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None