DNA

Part:BBa_K1323018

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)

oriV from the Staphylococcus aureus pSK41 Plasmid

This construct contains the origin of replication (oriV) from pSK41, a multiresistance plasmid from Staphylococcus aureus. This plasmid is low copy number and undergoes theta-mode of replication (Berg et al., 1998). pSK41 was first found in methicillin-resistant S. aureus (Kwong, 2006). The oriV is part of a replication region that spans a 1.25 kb section (nt 12916-14164) on the pSK41.

This part was DNA synthesized.



Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Berg, T. et al. (1998) Complete nucleotide sequence of pSK1: Evolution of staphylococcal conjugative multiresistance plasmids. J. Bacteriol. 180:4350-4359.

Kwong, S., Skurray, R., Firth, N. (2006). Replication control of staphylococcal multiresistance plasmid pSK41: an antisense RNA mediates dual-level regulation of Rep expression. Journal of Bacteriology, 188(12), 4404-4412.


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