Coding

Part:BBa_K1323013

Designed by: Julia Manalil   Group: iGEM14_Waterloo   (2014-10-04)

aadD - Kanamycin Resistance Gene Coding Sequence

This coding sequence is for the antibiotic kanamycin. The sequence of this gene is obtained from pKAN vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The working concentration of kanamycin is 250 µg/mL for aadD expressed on a plasmid and 75 µg/mL for aadD on chromosome (Bose et al., 2013).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 561
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 385


References

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.


[edit]
Categories
//cds/selectionmarker/antibioticresistance
Parameters
None