Designed by: Julia Manalil Group: iGEM14_Waterloo (2014-10-04)
aadD - Kanamycin Resistance Gene Coding Sequence
This coding sequence is for the antibiotic kanamycin. The sequence of this gene is obtained from pKAN vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The working concentration of kanamycin is 250 µg/mL for aadD expressed on a plasmid and 75 µg/mL for aadD on chromosome (Bose et al., 2013).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal BglII site found at 561
- 23COMPATIBLE WITH RFC
- 25INCOMPATIBLE WITH RFCIllegal AgeI site found at 172
- 1000INCOMPATIBLE WITH RFCIllegal SapI.rc site found at 385
Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.