Hfq: Expression cassette under a xylose inducible promoter
Hfq is a protein which enhances sRNA complementation to its mRNA target - it does so by binding AU-rich and poly-A-rich sequences. sRNAs which associate with Hfq have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) E. coli contain the Hfq gene in their chromosomes - other species, including Staphylococcus sp., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in S. epidermidis using JCat software.
This part was DNA synthesized by Bio Basic.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal BamHI site found at 1476
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Yoo, S, M., Na, D., Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocol 8(9), 1694-1707.