Designed by: Julia Manalil Group: iGEM14_Waterloo (2014-10-07)
sgRNA (1): CRISPR Silencing of YFP
Single guide RNA (sgRNA) 1 is an RNA oligo that associates with dCas9 BBa_K1323002 and guides the CRISPRi complex to the coding strand of the YFP BBa_K1323010 at nt position 43 - 62. sgRNA is a fusion of crRNA (CRISPR RNA) and tracrRNA (trans-activation crRNA) - the 20 bp sequence is complementary to the target sequence and is linked to a hairpin loop that is similar to tracrRNA. When the sgRNA-dCas9 complex binds to the target sequence, it is expected to act as a programmable repressor and silence expression of the target gene.
This part was DNA synthesized by Bio Basic.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC