Part:BBa_K1321312
Anderson J23103 in pSEVA 331-Bb
This is Anderson promoter J23103 driving the expression of Elowitz RBS-mFRP in pSEVA331-Bb plasmid backbone (part BBa_K1321300 and is a member of the K. rhaeticus genetic engineering toolkit (parts BBa_K1321295 - BBa_K1321332).
Komagataeibacter toolkit was designed to ease genetic engineering of cellulose-based biomaterials using the cellulose synthesizing bacterium Komagataeibacter rhaeticus iGEM (parts Bba_K1321305 and BBa_K1321306). As no registry parts had been tested in K. rhaeticus, the aim of this toolkit was to determine the parts usable in K. rhaeticus and to characterize them in this host. pSEVA331-Bb is a non-standard broad host range plasmid capable of replication in K. rhaeticus and E. coli (a shuttle vector) and was selected because the registry's standard plasmid backbone pSB1C3 can not be used for K. rhaeticus engineering.
NOTE: Because the registry's standard plasmid backbone pSB1C3 is not capable of replication in Komagataeibacter or Gluconacetobacter species, the Komagataeibacter genetic engineering toolkit is housed mainly in pSEVA331-Bb. pSEVA331-Bb is a non-standard backbone, which therefore can't be quality controlled by and maintained in the Registry. However, in order to make the Komagataeibacter toolkit available for the synthetic biology community, Imperial iGEM 2014 team has made it freely available in AddGene or upon request.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 3776
Illegal SpeI site found at 37
Illegal PstI site found at 920 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3776
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NotI site found at 913
Illegal NotI site found at 3782 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3776
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 3776
Illegal SpeI site found at 37
Illegal PstI site found at 920 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 3776
Illegal XbaI site found at 3791
Illegal SpeI site found at 37
Illegal PstI site found at 920
Illegal NgoMIV site found at 2095
Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
None |