Coding

Part:BBa_K1321123

Designed by: Gabriella Santosa   Group: iGEM14_Imperial   (2014-10-09)

Metallothionein SmtA + linker-CBDcipA-linker

Metallothionein (metal binding peptide) SmtA fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous C and N-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-SmtA-part-table.PNG

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 319
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 319
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 319
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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