Composite

Part:BBa_K1320000

Designed by: Will Reiber   Group: iGEM14_Gaston_Day_School   (2014-09-18)

Cd sensitive promoter with Ogr activator, PO promoter, and GFP

The cadmium sensitive promoter catalyzes the production of the Ogr activator. The activator triggers the PO promoter causing the production of GFP at greater levels than the cadmium promoter alone.

This part has detectable GFP production at 2.5mM Cadmium nitrate. This is a significant increase over the 10mM detection limit for the 2012 Cd detector K824008.

2014 Cd Detector Construct Results
X axis is Cd concentration (M)
Y axis is fluorescence / OD
Cadmium_graph.jpg
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1200


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Categories
Parameters
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