Coding

Part:BBa_K1317004

Designed by: Mounir Benkoulouche   Group: iGEM14_Bordeaux   (2014-10-07)

Fusion protein RFP + Elastin like polypeptide (ELP)

The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process.

Link on our wiki: [http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317004 http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317004]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 894
    Illegal AgeI site found at 1006
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None