Part:BBa_K1317002

CDS of silk-like protein (SLP)

This part is the coding sequence for the silk like polypeptide. This sequence was assembled from a consensus of the MaSp1 from spiders. This protein, in spiders, allows resistance and elasticity used for spinning the webs. The synthetic gene encodes a synthetic protein "silk-like". The repeated aminoacids allow to retrieve these properties of resistance, resilience and elasticity, but with a minimal pattern of the original protein, which is more suited for downstream applications. It can be used to produce wire presenting these properties after wet-spinning.

Link on our wiki: [http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317002 http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317002]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 312
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Römer L. et Scheibel T. The elaborate structure of spider silk : Structure and function of a natural high performance fiber (2008) Prion, 2(4), 154-61