Regulatory

Part:BBa_K1316004

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-08-24)

ybiJ promoter

Promoter of the ybiJ gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.[1]

Characterisation

The functionality of the ybiJ promoter is proven by the production of mKate2 in response to 2,4-DNT by the BioBrick BBa_K1316007. This promoter, besides, seems to be more sensitive than the yqjF promoter regarding its induction with 2,4-DNT.


References

[1] S. Yagur-Kroll, S. Belkin et al., “Escherichia Coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 381
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None