Reporter

Part:BBa_K1316000

Designed by: Joan Cortada Garcia   Group: iGEM14_TU_Delft-Leiden   (2014-08-24)

mKate2 protein

mKate2 is a far-red fluorescent protein used with reporting purposes. mKate2 codon usage is optimised for high expression in mammalian cells (humanised). It is, nevertheless, suitable for propagation in E. coli.

Characterisation

This Basic part was used for the construction of the final BioBricks BBa_K1316003, BBa_K1316005, BBa_K1316007, BBa_K1316008 and BBa_K1316009. The functionality of this part is proven by the red fluorescence detected on the characterisation of BioBrick BBa_K1316003 (http://parts.igem.org/Part:BBa_K1316003).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 454
    Illegal BsaI.rc site found at 643


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Parameters
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