Part:BBa_K1315004

ydeH (dgcZ)

This is the coding sequence for the zinc-sensory diguanylate cyclase (YdeH). It produces c-di-GMP, which however might prove toxic to E.coli cells when tried to be added. The gene was used to provide some extra cyclic-di-GMP to inhibit Clp(BBa_K1315005).

Usage and Biology

Diguanylate cyclases produce cyclic diGMP (c-di-GMP), a secondary messenger. Diguanylate Cyclase (DgcZ), also called YdeH, is present in Escherichia coli and the stimulus that controls it is Zinc.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 775
  Illegal SapI.rc site found at 800